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RSorder Easter Eggs Hunting: Take Free RS Gold for Sale and $15 Voucher on Apr.13 - Printable Version

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RSorder Easter Eggs Hunting: Take Free RS Gold for Sale and $15 Voucher on Apr.13 - rs3gold5 - 04-09-2020

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This study monitored the dynamics and diversity of the human faecal 'Atopobium cluster' over a 3 month period using a polyphasic approach. Fresh faecal samples were collected fortnightly from 13 healthy donors (6 males and 7 females) aged between 26 and 61 years. Fluorescence in situ hybridization was used to enumerate total (EUB338mix) and 'Atopobium cluster' (ATO291) bacteria, with counts ranging between 1.12  1011 and 9.95  1011, and 1.03  109 and 1.16  1011 cells (g dry weight faeces) 1, respectively. The 'Atopobium cluster' population represented 0.2 22 % of the total bacteria, with proportions donor dependent. Denaturing gradient gel electrophoresis (DGGE) using 'Atopobium cluster' specific primers demonstrated faecal populations of these bacteria were relatively stable, with bands identified as Collinsella aerofaciens, Collinsella intestinalis/Collinsella stercoris, Collinsella tanakaei, Coriobacteriaceae sp. PEAV3 3, Eggerthella lenta, Gordonibacter pamelaeae, Olsenella profusa, Olsenella uli and Paraeggerthella hongkongensis in the DGGE profiles of individuals. Colony PCR was used to identify 'Atopobium cluster' bacteria isolated from faeces (n = 224 isolates). 16S rRNA gene sequence analysis of isolates demonstrated Collinsella aerofaciens represented the predominant (88 % of isolates) member of the 'Atopobium cluster' found in human faeces, being found in nine individuals. Eggerthella lenta was identified in three individuals (3.6 % of isolates). Isolates of Collinsella tanakaei, an 'Enorma' sp. and representatives of novel species belonging to the 'Atopobium cluster' were also identified in the study. Phenotypic characterization of the isolates demonstrated their highly saccharolytic nature and heterogeneous phenotypic profiles, and 97 % of the isolates displayed lipase activity.
If you're diagnosed with breast cancer, you and your doctor will need to know what type it is and how advanced it is. A check of your lymph nodes can tell if the disease has spread. Other tests give an idea of what treatments may work best for you, and still others predict the likelihood that your cancer will come back after treatment.

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[Image: easter_egg_hunting_seo.jpg]

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